Allina Health Laboratory a part of Abbott Northwestern Hospital

  



  
    

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Test Name:p53 by IHC
Test Number:12376 - Technical only; 12379 - Technical & Interpretation
Collect:Formalin-fixed, paraffin embedded (FFPE) tissue block
Container:FFPE tissue block
Processing:Submit processed tissue block or tissue section mounted on a charged, unstained slide
Transport/Stability:Ambient (preferred)
Alternate Names:IHC
LAB12376
LAB12379
Performing Lab:AHL - Immunohistochemistry
Days Set Up:Mo - Fr
Expected TAT:1 - 2 days
Ref. Ranges:If requested, an interpretive report will be provided
All IHC stains will include a positive control tissue
Collection/
Processing Details:

Specifications:
  • P53 is a transcription factor that regulates cell growth and inhibits cells from entering S-phase
  • P53 is a DNA-binding phosphoprotein coded for by a tumor suppressor gene located on the short arm of chromosome 17
  • Mutations of p53 or functional inactivation with intact p53 genes are common in many cancers, leading to loss of normal growth regulation
  • Although p53 is present in small amounts in normal cells (not usually detected by routine immunohistochemistry), mutations resulting in a prolonged half-lives lead to accumulations that are detected by immunohistochemistry
  • Over-expression is associated with a poor prognosis in many cancers
Staining pattern:
  • Nuclear staining
Applications:
  • P53 over-expression is present in many different malignancies, such as colon, stomach, bladder, breast, lung, testes, melanomas, sarcomas, etc.
References:
  1. Bartek J et al: Aberrant expression of the p53 oncoprotein is a common feature of a wide spectrum of human malignancies. Oncogene 1991; 6: 1699-703.

Method:Immunohistochemical staining
Microscopic examination
CPT Codes:88342 - 1st stain
88341 - each additional stain
Date Created:07/19/2017
Revised Date:10/19/2018