Submit processed tissue block or tissue section mounted on a charged, unstained slide
AHL - Immunohistochemistry
Days Set Up:
Mo - Fr
1 - 2 days
If requested, an interpretive report will be provided All IHC stains will include a positive control tissue
Collection/ Processing Details:
p63 is made of two domains, including p63 and p40 isomers. Current antibodies for p63 recognize both isomers. And, although sensitive for identifying squamous cell carcinomas, this antibody is non-specific, and can also mark adenocarcinomas
The p40 isomer selectively expressed in lung squamous cell carcinomas
p40 is equivalent to p63 in sensitivity for lung SCC, but is markedly more specific
p40 stains fewer lung adenocarcinoma cases compared to p63; 0.8% of lung adenocarcinomas stained in one study 1
Most urothelial cancers are p40 positive (85%)
95% of skin SCC and basal cell carcinomas are p40 positive
p40 is equivalent to p63 in staining myoepithelial cells of breast and prostate
Recent study showed p40-positive cases were as follows: 153 (96.8%) of 158 SCCs, 7 (4.6%) of 152 adenocarcinomas, 0 (0%) of 50 carcinoid tumors, 4 (3.6%) of 107 large cell neuroendocrine carcinomas, 1 (1.5%) of 68 small cell lung carcinomas, and 1 (2.4%) of 41 mesotheliomas2
p40 is a useful marker for excluding neuroendocrine carcinomas and mesotheliomas
Non-SCC's show focal and weak staining as compared to SCC which is typically diffuse and strong
P40 is useful in discriminating between primary adnexal carcinomas and cutaneous metastases 4
Tascha D et al: An Immunohistochemical Analysis of a Newly Developed,
Mouse Monoclonal p40 (BC28) in Lung, Bladder, Skin, Breast, Prostate, and Head and Neck CancersArch Pathol Lab Med. doi: 10.5858/arpa.2013-0342-OA.
Tatsumori et al: p40 is the Best Marker for Diagnosing Pulmonary Squamous Cell Carcinoma: Comparison With p63, Cytokeratin 5/6, Desmocollin-3, and Sox2. Appl Immunohistochem Mol Morphol 2014;22:377-382).
Ao MH et al: The utility of a novel triple marker (combination of TTF1, Napsin A, and p40) in the subclassification of non-small cell lung cancer. Human Pathology (2014) 45, 926-934.
Lee JJ et al: p40 exhibits better specificity than p63 in distinguishing primary skin adnexal carcinomas from cutaneous metastases. Human Pathology (2014) 45, 1078-1083.
Butnor KJ et al: p40 (△Np63) and keratin 34βE12 provide greater diagnostic accuracy than p63 in the evaluation of small cell lung carcinoma in small biopsy samples. Human Pathology (2013) 44, 1479-1486.