Submit processed tissue block or tissue section mounted on a charged, unstained slide.
Transport/Stability:
Ambient (preferred)
Alternate Names:
IHC
LAB12376
LAB12379
Performing Lab:
AHL - Immunohistochemistry
Days Set Up:
Mo - Fr
Expected TAT:
1 - 2 days
Ref. Ranges:
If requested, an interpretive report will be provided All IHC stains will include a positive control tissue
Collection/ Processing Details:
Specifications:
Arginase-1 is a urea cycle metalloenzyme that is expressed in liver
This antibody is a sensitive and specific marker that can be used to confirm hepatocellular differentiation and exclude other possible primaries
Staining patterns:
Strong diffuse cytoplasmic staining; patchy nuclear staining can also be seen
Applications:
Arginase-1 reactivity is seen in the vast majority of well-differentiated hepatocellular carcinomas (HCC), but may be absent in poorly differentiated HCCs
This antibody is more sensitive than HepPar-1 in identifying HCC (table 2 2)
This antibody is very useful with Glypican 3 in identifying scirrhous HCC (more useful than HepPar-1)
One study showed rare non-hepatic tumors with Arginase reactivity (cholangiocarcinoma and prostatic adenocarcinoma; 2)
References:
Krings G et al: Immunohistochemical pitfalls and the importance of glypican 3 and arginase in the diagnosis of scirrhous hepatocellular carcinoma. Modern Pathology (2013) 26, 782–791.
Yan, BC et al: A New Immunohistochemical Marker of Hepatocytes and Hepatocellular Neoplasms. Am J Surg Pathol 2010;34:1147–1154).
Ordonez NG: Arginase-1 is a novel immunohistochemical marker of hepatocellular differentiation. Adv Anat Pathol 2014;21:285–290.
Staining patterns: