Allina Health Laboratory a part of Abbott Northwestern Hospital



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Test Name:Arginase-1 by IHC
Test Number:12376 - Technical only; 12379 - Technical & interpretation
Collect:Formalin-fixed, paraffin embedded (FFPE) tissue block.
Container:FFPE tissue block
Processing:Submit processed tissue block or tissue section mounted on a charged, unstained slide.
Transport/Stability:Ambient (preferred)
Alternate Names:IHC
Performing Lab:AHL - Immunohistochemistry
Days Set Up:Mo - Fr
Expected TAT:1 - 2 days
Ref. Ranges:If requested, an interpretive report will be provided
All IHC stains will include a positive control tissue
Processing Details:

  • Arginase-1 is a urea cycle metalloenzyme that is expressed in liver
  • This antibody is a sensitive and specific marker that can be used to confirm hepatocellular differentiation and exclude other possible primaries
Staining patterns:
  • Strong diffuse cytoplasmic staining; patchy nuclear staining can also be seen
  • Arginase-1 reactivity is seen in the vast majority of well-differentiated hepatocellular carcinomas (HCC), but may be absent in poorly differentiated HCCs
  • This antibody is more sensitive than HepPar-1 in identifying HCC (table 2 2)
  • This antibody is very useful with Glypican 3 in identifying scirrhous HCC (more useful than HepPar-1)
  • One study showed rare non-hepatic tumors with Arginase reactivity (cholangiocarcinoma and prostatic adenocarcinoma; 2)
  1. Krings G et al: Immunohistochemical pitfalls and the importance of glypican 3 and arginase in the diagnosis of scirrhous hepatocellular carcinoma. Modern Pathology (2013) 26, 782–791.
  2. Yan, BC et al: A New Immunohistochemical Marker of Hepatocytes and Hepatocellular Neoplasms. Am J Surg Pathol 2010;34:1147–1154).
  3. Ordonez NG: Arginase-1 is a novel immunohistochemical marker of hepatocellular differentiation. Adv Anat Pathol 2014;21:285–290.

Method:Immunohistochemical staining
Microscopic examination
CPT Codes:88342 - 1st stain
88341 - each additional stain
Date Created:05/01/2017
Revised Date:10/17/2018