Anaerobes are part of the endogenous flora of the body, so it is important to culture only specimen from sites that do not have normal anaerobic flora.
Sources appropriate for anaerobic culture include:
Abcess aspirate (including rectal, oral and vaginal), body fluid, bronchoscopy, Cerebral spinal fluid (CSF), IUD for Actinomyces species, placent tissue from c-section, sinus aspirate/swab, surgical specimens not contaminated with bowel flora, tissue, transtracheal aspirate or urine collected by suprapubic aspirate.
Swab: ESwab regular or mini-tip with Amies
Fluid: Port-A-Cul anaerobic vial Tissue: ESwab transport tube in the liquid without the swab
Deliver within 48 hours
ESwab: Ambient or Refrigerated
AHL- Microbiology; M
Days Set Up:
Mo - Su
Preliminary: 2 days
Final: 5 -7 days
Collection/ Processing Details:
Specimen MUST be in ESwab or anaerobic transport media. Allina Health metro hospital collections may be sent directly to the lab in a capped syringe with air expressed out within one hour of collection.
The follow specimens are unacceptable for anaerobic culture:
Colostomy discharge, decubitus ulcer, fecal/rectal swab, foley cath tips, oral swab, placenta from a vaginal delivery, skin swab, sputum, BAL or bronchoscopy specimens NOT collected by protected brush, specimens from sites contaminated with fecal flora, superficial wound specimens, throat, nasal or nasopharyngeal (NP) swabs, vaginal/cervical swabs, voided or catheterized urine and swabs not in anaerobic transport media.
Identification, susceptibilities and serogrouping will be performed on all microbiology isolates considered by the laboratory to be significant. See the Allina Health Laboratory fee schedule for specific tests and CPT codes.