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Test Name:Anaerobic Culture
Test Number:6554
Collect:Anaerobes are part of the endogenous flora of the body, so it is important to culture only specimen from sites that do not have normal anaerobic flora.

Sources appropriate for anaerobic culture include:
Abscess aspirate (including rectal, oral and vaginal), body fluid, bronchoscopy, Cerebral spinal fluid (CSF), IUD for Actinomyces species, placental tissue from C-section delivery, sinus aspirate/swab, surgical specimens not contaminated with bowel flora, tissue, transtracheal aspirate or urine collected by suprapubic aspirate.
Container:Swab: ESwab regular or mini-tip with Amies
Fluid: Port-A-Cul anaerobic vial
Tissue: ESwab transport tube in the liquid without the swab
Processing:Deliver within 48 hours
Transport/Stability:Port-A-Cul: Ambient
ESwab: Ambient or Refrigerated
Alternate Names:ANC
87075.0
Performing Lab:AHL- Microbiology; M
Days Set Up:Mo - Su
Expected TAT:Preliminary: 2 days
Final: 5 -7 days
Ref. Ranges:N/A
Collection/
Processing Details:
Specimen MUST be in ESwab or anaerobic transport media.
Allina Health metro hospital collections may be sent directly to the lab in a capped syringe (no needles) with air expressed out within one hour of collection.

The follow specimens are unacceptable for anaerobic culture:
Colostomy discharge, decubitus ulcer, fecal/rectal swab, foley cath tips, oral swab, placenta from a vaginal delivery, skin swab, sputum, BAL or bronchoscopy specimens NOT collected by protected brush, specimens from sites contaminated with fecal flora, superficial wound specimens, throat, nasal or nasopharyngeal (NP) swabs, vaginal/cervical swabs, voided or catheterized urine and swabs not in anaerobic transport media.

Notes:
  • If specimen quantity is limited, the entire specimen can be placed in the anaerobic container. Aerobic, anaerobic, fungus and AFB cultures can all be performed on specimens in anaerobic transport containers.
  • Order Anaerobic Culture and the appropriate aerobic culture (ex, Body Fluid Culture, Tissue Culture, etc.)

  • Needle Aspiration (preferred method)
    Container: Port-A-Cul anaerobic culture vial (Lawson #105866)

    1. Remove flip cap from Port-A-Cul anaerobic culture vial. Disinfect rubber stopper of vial with 70% alcohol prep.
    2. Using aseptic technique, aspirate specimen with a sterile syringe and needle. Expel air from syringe.
    3. Inject fluid slowly through rubber stopper into Port-A-Cul anaerobic culture vial.
    4. Label specimen with patient’s name, ID#, specimen source, and date and time of collection.

    Swab Collection
    Container: ESwab regular with liquid Amies collection kit (Lawson # 254730). Contains a transport tube with 1 mL liquid Amies medium and an ESwab applicator.

    1. Open and remove the tube and swab applicator.
    2. Collect specimen. Do not touch the area below the red breakpoint line.
    3. Aseptically remove cap from tube.
    4. Insert swab into tube and bend shaft against tube at colored line to break shaft.
    5. Replace cap on the tube and secure tightly.
    6. Label specimen with patient’s name, ID#, specimen source, and date and time of collection.

    Tissue Collection (preferred method)
    Container: ESwab regular with liquid Amies Collection Kit (Lawson #254730). Contains a transport tube with 1 mL liquid Amies medium and an ESwab applicator.

    1. Open and remove the transport tube. The ESwab applicator is not needed.
    2. Aseptically remove cap from tube.
    3. Using aseptic technique, place the tissue in the liquid in the transport tube.
    4. Replace cap on the tube and secure tightly.
    5. Label specimen with patient’s name, ID#, specimen source, and date and time of collection.
Method:Culture
CPT Codes:87075

Identification, susceptibilities and serogrouping will be performed on all microbiology isolates considered by the laboratory to be significant. See the Allina Health Laboratory fee schedule for specific tests and CPT codes.
Supply Connection:Anaerobic Swab Transport
Date Created:11/30/2001
Revised Date:03/27/2018