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Test Name:Amyloid A by IHC
Test Number:12376 - Technical only; 12379 - Technical & interpretation
Collect:Formalin-fixed, paraffin embedded (FFPE) tissue block.
Container:FFPE tissue block
Processing:Submit processed tissue block or tissue section mounted on a charged, unstained slide.
Transport/Stability:Ambient (preferred)
Alternate Names:Serum Amyloid A
IHC
SSA
LAB12376
LAB12379
Performing Lab:AHL - Immunohistochemistry
Days Set Up:Mo - Fr
Expected TAT:1 - 2 days
Ref. Ranges:If requested, an interpretive report will be provided
All IHC stains will include a positive control tissue
Collection/
Processing Details:

Specifications:
  • This antibody labels amyloid A (AA) in tissues and can be used to classify AA-amyloidosis.
  • This antibody does not react with other amyloid types, such as Alzheimer’s amyloid plaques
  • SAA can be used in a panel with glutamine synthetase and beta catenin to help differentiate between hepatic adenomas (HA) and focal nodular hyperplasia (FNH)


Staining patterns:
  • Strong cytoplasmic staining.

Applications:
  • Most inflammatory HAs show strong SAA staining, which can be patchy or diffuse (>90%)
  • SAA is negative in most (83%) of FNH cases (rare cases show patchy or diffuse staining)
References:
  1. Linke RP: Monoclonal antibodies against amyloid fibril protein AA. Production, specificity and use for immunohistochemical localization and classification of AA-type amyloidosis. J Histochem Cytochem 1984;32:322-8.
  2. Shafizadeh N et al: Diagnosis of well-differentiated hepatocellular lesions: role of immunohistochemistry and other ancillary techniques. Adv Anat Pathol 2011;18:438-445).
  3. Joseph NM et al: Diagnostic utility and limitations of glutamine synthetase and serum amyloid-associated protein immunohistochemistry in the distinction of focal nodular hyperplasia and inflammatory hepatocellular adenoma. Modern Pathology (2014) 27, 62–72; doi:10.1038/modpathol.2013.114.

Method:Immunohistochemical staining
Microscopic examination
CPT Codes:88342 - 1st stain
88341 - each additional stain
Date Created:03/02/2016
Revised Date:10/17/2018