Allina Health Laboratory a part of Abbott Northwestern Hospital



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Test Name:PML-RARA transcript detection for Acute Promyelocytic Leukemia, quantitative
Test Number:13557
Collect:7 mL EDTA whole blood - lavender
Minimum Volume:3 mL whole blood
1.0 mL bone marrow
Alternate Collect:7mL Na heparin whole blood - green
7 mL ACD-A whole blood - yellow
3.0 mL Bone Marrrow
Cell pellet
Container:Lavender (EDTA), green (Na heparin) or yellow (ACD-A) topped tube, or
Sterile container
If specimen is to be stored prior to shipment, store at 2 - 8°C
Specimens must arrive at LabCorp within 48 hours of collection.
Alternate Names:15;17 Translocation Detection by RT-PCR

Performing Lab:LabCorp RTP (510840); R-LC
Days Set Up:Mon - Fri
Expected TAT:4 - 9 days
Ref. Ranges:An interpretive report will be provided
Processing Details:
Useful for: The translocation t(15;17)(q22;q21) is the prototype rearrangement found in the vast majority of acute promyelocytic leukemia (APL), being found in >95% of APL cases. In this chromosomal rearrangement, the retinoic acid receptor (RARA) gene on chromosome 17 is fused with the PML gene on chromosome 15. There are three common breakpoints within the PML gene, bcr1 (intron 6), bcr2 (exon 6), and bcr3 (intron 3). All breakpoints fuse a portion of the PML gene to a consistent breakpoint region within the RARA gene. This assay will detect the PML-RARA transcripts associated with the bcr1, bcr2, and bcr3 breakpoints using real-time RT-PCR in order to assist in the diagnosis and monitoring of APL. The results are reported as a normalized ratio of %PML- RARA copies/ABL1 copies. In vitro studies have indicated that this assay has an analytical sensitivity that allows for the detection of 0.001% PML-RARA/ABL1.

Method:TaqMan® quantitative polymerase chain reaction (Q-PCR)
CPT Codes:81315
Date Created:04/10/2019
Revised Date:07/15/2019