Allina Health Laboratory a part of Abbott Northwestern Hospital

  



  
    

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Test Name:Acanthamoeba species molecular detection, PCR, ocular
Test Number:994
Collect:The preferred specimen for Acanthomoeba PCR from an ocular source is corneal scraping or biopsy.
Alternate Collect:CNS tissue, contact lens solution, cerebral spinal fluid (CSF)
Container:Screw Cap Vial with Sterile Saline
Processing:Submit entire specimen
Transport/Stability:Refrigerated - 7 days
Alternate Names:MSO
LAB994
Performing Lab:Mayo Clinic Laboratories (ACARP); R
Days Set Up:Mo - Sa
Expected TAT:3 - 4 days
Ref. Ranges:Negative
Collection/
Processing Details:
Specimens collected using wooden shafted swabs will be canceled.
Fluorescein dye may interfere with testing and should not be used prior to collection; specimens containing fluorescein dye will be canceled.
Submit only 1 of the following specimens:

Specimen Type: Tissue: Fresh
Sources: Ocular
Container/Tube: Sterile container
Specimen Volume: 5-10 mm
Collection Instructions: Submit tissue in a sterile container with 1 mL of sterile saline, minimal essential media (MEM), or viral transport media.

Specimen Type: Tissue: Formalin-fixed paraffin-embedded (FFPE)
Sources: Ocular
Container/Tube: Sterile container
Specimen Volume: 5-10 mm
Collection Instructions: Cut tissue into five 10-micron sections and place in a sterile container.

Specimen Type: Scrapings/Swabs
Sources: Eye, ocular, cornea
Container/Tube: Sterile container
Specimen Volume: 1 mL
Collection Instructions:
1. Collect corneal scrapings using a scalpel or other sharp device to remove the outer layer of cells from the eye.
2. Swish the collection device in 1 mL of sterile saline, minimal essential media (MEM) or viral transport media.
3. Remove the collection device from the collection container before submitting to the lab.
4. Specimens containing scalpel blades will be canceled.
Additional Information: Swabs are not the preferred specimen for this test and may yield false negative results.

Specimen Type: Contact lenses
Container/Tube: Sterile container
Specimen Volume: Entire collection
Collection Instructions:
1. Place entire contact lens in a sterile container with 1 mL sterile saline, contact lens solution, viral transport media, or minimal essential media (MEM).
2. Right and Left lenses must be submitted individually using separate sterile containers for each lens. Alternatively, lenses may be submitted in separate chambers of a contact lens case. Separate orders must be created for each lens.
3. Indicate Right or Left in the specimen source.

Specimen Type: Contact lens solution
Container/Tube: Sterile container
Specimen Volume: 1 mL solution

Specimen Type: Contact lens cases without lenses
Container/Tube: Sterile container
Specimen Volume: 1 mL solution or entire case
Additional Information:
1. Depending on the type of case submitted, it may be necessary to test right and left chambers individually. Separate orders must be created for each chamber.
2. Indicate Right or Left in the specimen source.
Method:Real-Time Polymerase Chain Reaction (PCR)/Taqman DNA Probe Hybridization
CPT Codes:87798
Supply Connection:Sterile Saline Tube
Date Created:04/26/2010
Revised Date:11/08/2018