0.5 mL clear Perchloric acid supernatant from potassium oxalate/sodium fluoride whole blood - grey
0.2 mL Note: this volume does not allow for repeat testing
Perchloric acid tube
See Collection/Processing details
Refrigerated (preferred) - 7 days
Ambient - 5 days
Frozen - 7 days
LabCorp Burlington (004788); R-NX
Days Set Up:
1 - 2 days
0.3 − 0.7 mg/dL
Collection/ Processing Details:
Useful for: Increased pyruvic acid levels have been associated with diabetes mellitus, vitamin deficiencies, uremia, congestive heart failure, liver diseases, muscular dystrophy, thiamine deficiency, and neoplastic conditions. Pyruvic acid is useful in assessing oxygen deprivation and provides an index of the severity of circulatory failure.
Draw blood into chilled gray-top tube.
Immediately after blood is drawn, add 2 mL of whole blood from the gray-top tube to 2 mL of 8% perchloric acid (7 mL of 70% perchloric acid diluted to 100 mL with deionized water). NOTE: Use 1:1 blood (ie, equal parts) to 8% perchloric ratio if less blood is obtained.
Shake mixture vigorously for about 30 seconds.
Refrigerate for five minutes to ensure complete protein precipitation.
Centrifuge 5 to 10 minutes at approximately 1500xg.
Immediately transfer the CLEAR SUPERNATANT to a transport tube. Label the transport tube as "SUPERNATANT" and send to the laboratory. NOTE: A second centrifugation of the supernatant may be necessary to obtain a clear protein-free solution.