Allina Health Laboratory a part of Abbott Northwestern Hospital



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Test Name:Pyruvic acid, whole blood
Test Number:994
Collect:0.5 mL clear Perchloric acid supernatant from potassium oxalate/sodium fluoride whole blood - grey
Minimum Volume:0.2 mL
Note: this volume does not allow for repeat testing
Container:Perchloric acid tube
Processing:See Collection/Processing details
Transport/Stability:Refrigerated (preferred) - 7 days
Ambient - 5 days
Frozen - 7 days
Alternate Names:Pyruvate

Performing Lab:LabCorp Burlington (004788); R-NX
Days Set Up:Tu, Th
Expected TAT:1 - 2 days
Ref. Ranges:0.3 − 0.7 mg/dL
Processing Details:
Useful for: Increased pyruvic acid levels have been associated with diabetes mellitus, vitamin deficiencies, uremia, congestive heart failure, liver diseases, muscular dystrophy, thiamine deficiency, and neoplastic conditions. Pyruvic acid is useful in assessing oxygen deprivation and provides an index of the severity of circulatory failure.

Draw blood into chilled gray-top tube.
Immediately after blood is drawn, add 2 mL of whole blood from the gray-top tube to 2 mL of 8% perchloric acid (7 mL of 70% perchloric acid diluted to 100 mL with deionized water). NOTE: Use 1:1 blood (ie, equal parts) to 8% perchloric ratio if less blood is obtained.
Shake mixture vigorously for about 30 seconds.
Refrigerate for five minutes to ensure complete protein precipitation.
Centrifuge 5 to 10 minutes at approximately 1500xg.
Immediately transfer the CLEAR SUPERNATANT to a transport tube. Label the transport tube as "SUPERNATANT" and send to the laboratory. NOTE: A second centrifugation of the supernatant may be necessary to obtain a clear protein-free solution.
Method:Pyruvate - lactate; spectrophotometry
CPT Codes:84210
Supply Connection:Perchloric acid tube
Date Created:05/23/2019
Revised Date:05/23/2019