Allina Health Laboratory a part of Abbott Northwestern Hospital

  



  
    

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Test Name:Protein S antigen
Test Number:994
Collect:3.0 mL Na citrate plasma - lt blue
Container:LabCorp screw-capped purple frozen plastic transport tube labelled as PPP Na Citrate plasma
Processing:Double spin, separate and freeze.
Transport/Stability:Frozen (strict)
Alternate Names:MSO
LAB994
Performing Lab:LabCorp Burlington (164517); R-NX
Days Set Up:Daily
Expected TAT:1 - 3 days
Ref. Ranges:Total: In newborns, total PS levels are lower than in adults (12% to 60%). Levels gradually reach adult ranges by six months of age
Collection/
Processing Details:
Useful for: Confirmation and characterization of protein S (PS) congenital deficiency

Platelet poor citrated plasma samples (PPP) should be obtained by double centrifugation. Blood should be collected in a blue-top tube containing 3.2% buffered sodium citrate. Evacuated collection tubes must be filled to completion to ensure a proper blood to anticoagulant ratio. The sample should be mixed immediately by gentle inversion at least six times to ensure adequate mixing of the anticoagulant with the blood. A discard tube is not required prior to collection of coagulation samples, except when using a winged blood collection device (ie, "butterfly"), in which case a discard tube should be used.

Centrifuge for 10 minutes and carefully remove 2/3 of the plasma using a plastic transfer pipette, being careful not to disturb the cells.
Deliver to a plastic transport tube, cap, and recentrifuge for 10 minutes. Use a second plastic pipette to remove the plasma, staying clear of the platelets at the bottom of the tube. Transfer the plasma into a LabCorp frozen purple tube with screw cap. Freeze immediately and maintain frozen until tested.

If the patient's hematocrit exceeds 55%, the volume of citrate in the collection tube must be adjusted.
Method:Total and Free PS antigens: latex immunoassay
Free PS: EIA after C4b-BP complexed PS is prepcipitated out with polyethylene glycol.
CPT Codes:85306
85305
Date Created:05/23/2019
Revised Date:05/23/2019