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Test Name:AFB culture & smear
Test Number:6617
Collect:See table below
Container:15 ml Sterile Falcon Tube or other sterile, leakproof container
ESwab or other bacterial culture swab (acceptable, but not preferred)
Processing:Submit entire specimen
Transport/Stability:Refrigerated - 2 weeks
Alternate Names:TB
Mycobacterium, AFC
Performing Lab:AHL - Microbiology; M
Days Set Up:Mo - Su
Expected TAT:Smear - 1 day
Culture - 6 weeks
Ref. Ranges:NA
Processing Details:
Specimen Recommended quantity Special instructions
Body fluid Adult: >10mL
Child (≤14 yrs): ≥1mL
If specimen might clot, place in a sterile sodium heparin tube (Lithium heparin and EDTA are unacceptable). Transport refrigerated.
Bone marrow As much as possible Collect aseptically in sodium heparin (Lithium heparin is unacceptable). Do not refrigerate; transport at ambient (room) temperature.
Broncial washing Adult: ≥5mL
Child (≤14 yrs): ≥1mL
Gastric 5 – 10mL Collect early morning fasting specimen on 3 consecutive days. Transport refrigerated.
Spinal fluid 3 – 10mL Transport refrigerated
Sputum 5 – 10mL Patient should rinse mouth out vigorously with water (not mouthwash), before producing sputum

Obtain at least three consecutive sputum specimens, each collected in 8-24 hr intervals, with at least one being an early morning specimen because respiratory secretions pool overnight.

Unacceptable : More than one specimen in an 8 hour period.
Transport refrigerated.
Stool Pea-sized amount Transport refrigerated
Swab/skin Swabs are the least desirable specimen for isolation of Mycobacteria. Whenever possible, tissue biopsies or aspirated material is recommended for skin lesions and other sources.
Transport refrigerated
Urine Adult: >40mL
Child (≤14 yrs): 10mL
Three (3) clean catch early morning specimens are recommended.
Transport refrigerated.
For blood, order AFB blood culture, 6591
Method:Culture and Acid Fast Stain
CPT Codes:87116

Identification, susceptibilities and serogrouping will be performed on all microbiology isolates considered by the laboratory to be significant. See the Allina Health Laboratory fee schedule for specific tests and CPT codes.
Date Created:11/30/2001
Revised Date:09/07/2018