Formalin-fixed, paraffin embedded tissue block. All IHC stains will include a positive control tissue on each slide.
FFPE tissue block
Submit processed tissue block
AHL - Immunohistochemistry
Days Set Up:
Mo - Fr
1 - 2 days
If requested, an interpretive report will be provided
Collection/ Processing Details:
Alpha-fetoprotein is a 64kD tumor-associated embryonal antigen synthesized during fetal life in the yolk sac, liver and gastrointestinal tract and produced by liver, yolk sac and some germ cell tumors of testicular and ovarian origin.
Cytoplasmic based staining.
Among hepatomas 10-70% have been reported as positive in most series. Among positive cases, the frequency of individual AFP-positive cells varies from 5-50% or more.
AFP positivity has also been reported in hepatoblastomas of childhood.
Primary cholangiocarcinoma is AFP negative.
Germ cell tumors:
Endodermal sinus tumors are usually positive for AFP.
Some embryonal carcinomas and teratomatous epithelium are also positive for AFP.
Seminomas/dysgerminomas, intratubular germ cell neoplasia and choriocarcinomas are negative for AFP.
Bladder tumors may occasionally produce AFP (especially if they are of urachal origin).
Tissue Localization of Alpha-Fetoprotein
in Malignant Germ Cell Tumors