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Test Name:AFP by IHC
Test Number:12376 - Technical only; 12379 - Technical & Interpretation
Collect:Formalin-fixed, paraffin embedded tissue block.
All IHC stains will include a positive control tissue on each slide.
Container:FFPE tissue block
Processing:Submit processed tissue block
Transport/Stability:Ambient (preferred)
Alternate Names:Alpha fetoprotein
Performing Lab:AHL - Immunohistochemistry
Days Set Up:Mo - Fr
Expected TAT:1 - 2 days
Ref. Ranges:If requested, an interpretive report will be provided
Processing Details:

  • Alpha-fetoprotein is a 64kD tumor-associated embryonal antigen synthesized during fetal life in the yolk sac, liver and gastrointestinal tract and produced by liver, yolk sac and some germ cell tumors of testicular and ovarian origin.

Staining patterns:
  • Cytoplasmic based staining.

  • Among hepatomas 10-70% have been reported as positive in most series. Among positive cases, the frequency of individual AFP-positive cells varies from 5-50% or more.
  • AFP positivity has also been reported in hepatoblastomas of childhood.
  • Primary cholangiocarcinoma is AFP negative.
Germ cell tumors:
  • Endodermal sinus tumors are usually positive for AFP.
  • Some embryonal carcinomas and teratomatous epithelium are also positive for AFP.
  • Seminomas/dysgerminomas, intratubular germ cell neoplasia and choriocarcinomas are negative for AFP.
  • Bladder tumors may occasionally produce AFP (especially if they are of urachal origin).

Tissue Localization of Alpha-Fetoprotein in Malignant Germ Cell Tumors

Tumor type Percent positive
Testis Seminoma 0
Teratoma 0
Embryonal carcinoma 47
Endodermal sinus tumor 50
Choriocarcinoma 0
Ovary Dysgerminoma 0
Immature teratoma 0
Embryonal carcinoma 70
Endodermal sinus tumor 100
Choriocarcinoma 0
Method:Immunohistochemical staining
Microscopic examination
CPT Codes:88342 - 1st stain
88341 - each additional stain
Date Created:03/02/2016
Revised Date:04/23/2018