3.0 mL EDTA whole blood - Lavender top, or
3.0 mL Bone Marrow in EDTA
3.0 mL ACD whole blood – Yellow top
3.0 mL Na Citrate whole blood – Lt Blue top
Lavender (EDTA), Yellow (ACD) or Lt Blue (Na Citrate) top tube
Submit entire tube, unspun
Janus Kinase 2
Tyrosine Kinase mutation
AHL - Molecular Diagnostics; D
Days Set Up:
Collection/ Processing Details:
Description: Detection of point mutation (G to T mutation in exon 12 resulting in the V to F amino acid change at position 617) of the human JAK2 gene. This mutation is associated with myeloproliferative disorders.
The Calreticulin Exon 9 Assay can be ordered as a reflex to a negative JAK2 V617F Mutation Detection. If ordered as a reflex, do not order the individual assay.
Only ONE (1) tube needs to be drawn for any combination of the following tests: Calreticulin Exon 9 Assay, Factor V Leiden Mutation, Factor 2 Mutation, JAK2 V617F Mutation Detection and Hereditary Hemochromatosis.
Multiplex, Allele-Specific PCR (AS-PCR) analyzed by capillary electrophoresis