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Test Name:Adrenocorticotropin by IHC
Test Number:12376 - Technical only; 12379 - Technical & interpretation
Collect:Formalin-fixed, paraffin embedded (FFPE) tissue block.
Container:FFPE tissue block
Processing:Submit processed tissue block or tissue section mounted on a charged, unstained slide.
Transport/Stability:Ambient (preferred)
Alternate Names:ACTH
IHC
LAB12376
LAB12379
Performing Lab:AHL - Immunohistochemistry
Days Set Up:Mo - Fr
Expected TAT:1 - 2 days
Ref. Ranges:If requested, an interpretive report will be provided
All IHC stains will include a positive control tissue
Collection/
Processing Details:

Specifications:
  • This antibody reacts with ACTH. It recognizes the corticotrophic cells of the pituitary gland. In normal pituitary gland, these are mostly contained in the central portion of the adenohypophysis and constitute 15-20% of the cells in the gland
Staining patterns:
  • Cytoplasmic based staining
Applications:
  • Immunoperoxidase staining for the presence of pituitary hormones helps distinguish pituitary adenomas and the rare primary pituitary carcinomas from other tumors occurring in the region of the sella (such as craniopharyngioma, metastatic carcinoma, meningioma, lymphoma, leukemia and plasmacytoma), none of which contain or produce pituitary hormones. Positive staining for specific pituitary hormones can divide the pituitary adenomas into subclasses based on the corresponding cell type present
  • Negative staining for pituitary hormones does not rule out a pituitary adenoma since some contain none of the known adenohypophyseal hormones
  • ACTH cell adenomas comprise 10-15% of all pituitary adenomas, including the endocrinologically silent corticotrophic adenomas (2%) unassociated with biochemical or clinical evidence of cortisol excess

Method:Immunohistochemical staining
Microscopic examination
CPT Codes:88342 - 1st stain
88341 - each additional stain
Date Created:06/02/2015
Revised Date:10/17/2018