Allina Health Laboratory a part of Abbott Northwestern Hospital



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Test Name:Prolactin by IHC
Test Number:12376 - Technical only; 12379 - Technical & Interpretation
Collect:Formalin-fixed, paraffin embedded (FFPE) tissue block
Container:FFPE tissue block
Processing:Submit processed tissue block or tissue section mounted on a charged, unstained slide
Transport/Stability:Ambient (preferred)
Alternate Names:PRL
Performing Lab:AHL - Immunohistochemistry
Days Set Up:Mo - Fr
Expected TAT:1 - 2 days
Ref. Ranges:If requested, an interpretive report will be provided
All IHC stains will include a positive control tissue
Processing Details:

  • This antibody reacts with prolactin hormone
  • It recognizes the prolactin-secreting cells of the pituitary gland (also termed lactotrophs or mammotrophs)
  • In the normal pituitary gland, these are mostly contained in the lateral wing of the adenohypophysis (posterolateral edges) and account for 15-25% of the cells of the gland
Staining pattern:
  • Cytoplasmic based staining
  • IHC staining for the presence of pituitary hormones helps distinguish pituitary adenomas and the rare primary pituitary carcinomas from other tumors occurring in the region of the sella (such as craniopharyngioma, metastatic carcinoma, meningioma, lymphoma, leukemia, and plasmacytoma), none of which contain or produce pituitary hormones
  • Positive staining for specific pituitary hormones can divide the pituitary adenomas into subclasses based on the corresponding cell type present
  • Negative staining for pituitary hormones does not rule out a pituitary adenoma since some contain none of the known adenohypophyseal hormones
  • Prolactinomas often show a characteristic globular immunohistochemical reaction for PRL in the region of the paranuclear Golgi

Method:Immunohistochemical staining
Microscopic examination
CPT Codes:88342 - 1st stain
88341 - each additional stain
Date Created:08/01/2017
Revised Date:10/19/2018