Submit processed tissue block or tissue section mounted on a charged, unstained slide
AHL - Immunohistochemistry
Days Set Up:
Mo - Fr
1 - 2 days
If requested, an interpretive report will be provided All IHC stains will include a positive control tissue
Collection/ Processing Details:
TTF-1 reacts with a transcription factor expressed in thyroid (follicular cells), lung (type II cells, Clara cells), and diencephalon
P40 is one of 10 p63 isoforms which is highly specific for squamous differentiation
Positive TTF-1 staining: Brown nuclear staining
Positive P40 staining: Red nuclear staining
This cocktail is intended to be used to assist in differentiating lung adenocarcinomas and lung squamous cell carcinomas. This TTF-1/P40 cocktail has a reported sensitivity of 77% and a specificity of 100% for lung adenocarcinomas. This TTF-1/P40coktail has a reported sensitivity of 93% and a specificity of 92% for lung squamous cell carcinomas
Other tumors reported to be positive for TTF-1 include neuroendocrine tumors (pulmonary and non-pulmonary origin), thyroid neoplasms, a subset of endometrial and cervical neoplasms, papillary nasopharyngeal carcinomas, and colorectal adenocarcinoma. Tumors which have been reported to rarely stain for TTF-1 in a subset of tumor cells include: cholangiocarcinoma, ovarian, gastric/esophageal, pancreatic ductal, urothelial, hepatocellular, salivary gland neoplasms including adenoid cystic carcinoma, and adrenal cortical carcinoma
Other tumors reported to be positive for the P40 clone used in this cocktail include bladder, breast, and prostate
Brown AF, et al. Tissue preserving antibody cocktails to differentiate primary squamous cell carcinoma, adenocarcinoma, and small cell carcinoma of lung. Arch Pathol Lab Med. 2013 Sep;137(9):1274-81.
Russel D, et al. Dual immunostaining TTF-1 and p40 for subcategorizing of nonsmall cell carcinoma of the lung in cytologic and surgical specimens.