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Test Name:Adenovirus DNA by Rapid PCR
Test Number:994
Collect:Multiple sample types; see Collection/Processing Details
Container:Dependent on specimen type; see Collection/Processing details
Processing:
Transport/Stability:Refrigerated (preferred) - 7 days
Frozen - 7 days
Alternate Names:MSO
LAB994
Performing Lab:Mayo Medical Labs (LADV/89074); R-NX
Days Set Up:Mo - Fr
Expected TAT:2 - 5 days
Ref. Ranges:None detected
Collection/
Processing Details:
Submit only 1 of the following specimens:

Body Fluid
0.5 mL of body fluid. Do not centrifuge. Send specimen refrigerated in a screw-capped, sterile vial. Specimen cannot be frozen. Maintain sterility and forward promptly. Specimens grossly contaminated with blood may inhibit the PCR and produce false-negative results. The high sensitivity of amplification by PCR requires the specimen to be processed in an environment in which contamination of the specimen by adenovirus DNA is not likely.

Respiratory Specimen
1 mL of bronchial washing, bronchoalveolar lavage, naso-pharyngeal aspirate or washing, sputum, or tracheal aspirate. Send specimen refrigerated in a screw-capped, sterile container. Specimen cannot be frozen. Maintain sterility and forward promptly.

Spinal Fluid
0.5 mL of spinal fluid. Do not centrifuge. Send specimen refrigerated in a screw-capped, sterile vial. Specimen cannot be frozen. Maintain sterility and forward promptly. Specimens grossly contaminated with blood may inhibit the PCR and produce false-negative results. The high sensitivity of amplification by PCR requires the specimen to be processed in an environment in which contamination of the specimen by adenovirus DNA is not likely.

Stool
1 g of fresh stool. Send specimen refrigerated in a plastic, screw-capped, sterile container. Maintain sterility and forward promptly.

Swab
Swab area with a culture transport swab; Calcium alginate-tipped swabs, wooden shafted swabs, Eswabs or transport swabs containing gel are not acceptable for PCR testing. Place swab back into swab cylinder. If a culture transport swab is not available, M4 or M5 media is acceptable. Send specimen refrigerated. Specimen cannot be frozen. Maintain sterility and forward promptly.

Tissue
Send fresh tissue refrigerated in a screw-capped, sterile container containing 1 mL to 2 mL of sterile saline or multi-microbe medium (M5) (Mayo supply T484). Specimen cannot be frozen. Maintain sterility and forward promptly.

Urine
1 mL from a random urine collection. Send specimen refrigerated in a screw-capped, sterile container. Specimen cannot be frozen. Maintain sterility and forward promptly.
Method:Real-Time Polymerase Chain Reaction (PCR)/DNA Probe Hybridization
CPT Codes:87798
Date Created:08/28/2008
Revised Date:02/15/2017